HG-SEM-1
Third-Party Tested · Certificate on File- Lot-matched certificate of analysis, identity and purity by HPLC and mass spec
- Ships within 1 business day from the U.S., with tracking
- Research use only. Not for human or animal use
- Certificate of analysis available on request
- ≥99% purity specification, HPLC & mass-spec verified at source
- Ships from a U.S. facility within 1 business day
- Secure checkout · $12.99 2-Day shipping, free over $1,000
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HG-SEM-1In your orderNN9535 is a synthetic 31-residue GLP-1 receptor agonist carrying two amino acid substitutions relative to native human GLP-1 and a fatty-acid chain conjugated at lysine 26. A 2015 paper in the Journal of Medicinal Chemistry examines the chemistry choices behind extending the molecule's circulating half-life through increased albumin binding affinity.
NN9535 is a long-acting GLP-1 receptor agonist peptide with a fatty-acid side chain that extends its half-life. It is the reference compound for GLP-1 receptor studies and a common comparator in metabolic research.
Common research applications include:
- GLP-1 receptor pharmacology
- Glucose-handling and insulin-signaling models
- Comparator studies for newer incretin peptides
Specifications
- Class: GLP-1 receptor agonist
- Form: lyophilized powder in a sealed glass vial
- Available sizes: 20 mg
- Purity: verified at 99% or higher by HPLC, identity confirmed by mass spectrometry
- Testing: every lot is third-party tested, with a lot-matched Certificate of Analysis available on request
- supplied by a U.S. lab
- Storage: keep sealed, cool, dry and away from light; refrigerate after reconstitution
For laboratory research use only. This product is not for human or veterinary use, is not a drug, food or cosmetic, and is not intended to diagnose, treat, cure or prevent any disease.
What the Literature Studies
Lau and colleagues described NN9535's discovery chemistry in a 2015 paper in the Journal of Medicinal Chemistry, framing the goal as designing a longer-acting GLP-1 analog by increasing albumin affinity while securing stability against metabolic degradation. The paper reports that NN9535 carries two substitutions relative to native GLP-1, Aib at position 8 and Arg at position 34, and is derivatized at lysine 26 with a fatty acid moiety and linker chemistry chosen for high albumin affinity alongside retained GLP-1 receptor potency. Measured GLP-1 receptor affinity was three-fold lower than the related compound liraglutide while albumin affinity increased; in mini-pigs, the paper reports a plasma half-life of 46.1 hours and a mean residence time of 63.6 hours, figures the authors present as the pharmacokinetic basis for a once-weekly research compound.
Specification and Handling
This lot is a 31-residue peptide with Aib at position 2 and Lys26 conjugated to a gamma-Glu-(AEEA)2-C18 diacid, C-terminal carboxylate, full sequence per the certificate of analysis. Molecular formula is C187H291N45O59 with a molecular weight of 4113.58. CAS number is 910463-68-2. Supplied as a lyophilized powder, purity verified at 99% or greater by HPLC, identity confirmed by mass spectrometry, certificate of analysis for the lot available on request. The powder is soluble in bacteriostatic water. Sealed vials are stored at -20°C, protected from light and moisture; reconstituted material is kept refrigerated and handled per standard laboratory procedure for this peptide class.
Buying Notes
NN9535 is available in 5 mg, 10 mg, 20 mg, 30 mg, and 50 mg lyophilized vials, covering a wide range of total peptide quantities for different research scales and budgets across this catalog's metabolic category. The certificate of analysis for each lot reports HPLC purity and a mass spectrometry match to the 4113.58 theoretical mass, confirming the sequence and fatty-acid conjugation for that lot. For receptor-class comparisons, see /learn/nn9535 for handling detail on this specific lot, and /blog/what-is-nn9535 for background on the single-receptor GLP-1 agonist class this compound belongs to.
Certificate of Analysis & Safety Data
Frequently Asked Questions
What two substitutions distinguish NN9535 from native GLP-1?
The 2015 Lau paper in the Journal of Medicinal Chemistry reports an Aib substitution at position 8 and an Arg substitution at position 34 relative to native human GLP-1. These substitutions, along with the lysine 26 fatty-acid conjugation, define the analog's chemistry.
What pharmacokinetic data does the 2015 paper report in mini-pigs?
The paper reports a plasma half-life of 46.1 hours and a mean residence time of 63.6 hours for NN9535 in mini-pigs. These figures are presented as the basis for the compound's extended circulating profile relative to other GLP-1 analogs studied at the time.
How does NN9535's GLP-1 receptor affinity compare with liraglutide's?
The 2015 paper measured NN9535's GLP-1 receptor affinity as three-fold lower than liraglutide's while reporting increased albumin affinity. This trade-off between receptor affinity and albumin binding is the central design choice described in the paper.
Why is NN9535 offered in five different vial sizes?
The range reflects the range of total peptide quantities researchers typically request for this single-receptor GLP-1 agonist. Each size shares the same CAS number, 910463-68-2, and purity grade.
What does the certificate of analysis verify for a NN9535 lot?
It reports HPLC purity at 99% or greater and a mass spectrometry check against the 4113.58 theoretical mass for the C187H291N45O59 formula. This confirms the peptide's identity and fatty-acid conjugation.
Is HG-SEM-1 third-party tested?
Yes. Every lot is tested for identity and purity at the source lab by HPLC and mass spectrometry against a ≥99% purity specification. A certificate of analysis for your lot is available on request.
Can I see the Certificate of Analysis before buying?
Yes. Ask us before you order and we will send the certificate of analysis for the current lot, documenting identity, purity, and analytical results.
What does 'Research Use Only' mean?
This product is supplied strictly for laboratory and research use only (RUO). It is not a drug, food, supplement, or cosmetic, is not approved by the FDA, and is not intended to diagnose, treat, cure, or prevent any disease, or for human or veterinary consumption.
How is it shipped and stored?
Orders ship within 1 business day from our U.S. facility with tracking. Store the lyophilized material at −20°C, protected from light and moisture.
